Choline-related supplements improve abnormal plasma methionine-
homocysteine metabolites and glutathione status in children with
cystic fibrosis.
Innis SM, Davidson AG, Melynk S, James SJ
Am J Clin Nutr 2007 Mar; 85(3):702-8.
BACKGROUND:
Liver triacylglycerol accumulation and oxidative stress are common in
cystic fibrosis (CF) and also occur in choline deficiency. Previously,
we showed an association between elevated plasma homocysteine, reduced
ratios of S-adenosylmethionine to S-adenosylhomocysteine (SAM:SAH) and
of phosphatidylcholine to phosphatidylethanolamine, and phospholipid
malabsorption in children with CF.
OBJECTIVE:
The objective was to address a possible relation between altered
methionine-homocysteine metabolism and choline metabolism in children
with CF.
DESIGN: Children with CF were assigned without bias to supplementation
with 2 g lecithin/d (n = 13), 2 g choline/d (n = 12), or 3 g betaine/d
(n = 10) for 14 d. Plasma concentrations of methionine, adenosine,
cysteine, cysteinyl-glycine, glutathione, glutathione disulfide
(GSSG), and fatty acids; SAM:SAH; and red blood cell phospholipids
were measured within each group of children with CF before and after
supplementation. Plasma from healthy children without CF (n = 15) was
analyzed to obtain reference data.
RESULTS:
Children with CF had higher plasma homocysteine, SAH, and adenosine
and lower methionine, SAM:SAH, and glutathione:GSSG than did children
without CF. Supplementation with lecithin, choline, or betaine
resulted in a significant increase in plasma methionine, SAM, SAM:SAH,
and glutathione:GSSG and a decrease in SAH (n = 35). Supplementation
with choline or betaine was associated with a significant decrease in
plasma SAH and an increase in SAM:SAH, methionine, and
glutathione:GSSG. Supplementation with lecithin or choline also
increased plasma methionine and SAM.
CONCLUSION:
We showed that dietary supplementation with choline-related compounds
improves the low SAM:SAH and glutathione redox balance in children
with CF.
The American journal of clinical nutrition [Am J Clin Nutr]
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